| Abstract |
Amniotic membrane-derived mesenchymal stem cells (AM-MSCs) possess significant proliferative and multilineage differentiation potential. However, prolonged in vitro culture and cryopreservation can negatively impact cell viability and expansion capacity. This study investigates the effects of growth factor supplementation on the proliferation of advanced-passage canine AM-MSCs (cAM-MSCs) before and after cryopreservation. cAM-MSCs were isolated from late-gestation canine fetuses, cultured in vitro, and supplemented with different growth factors: fibroblast growth factor 2 (FGF-2), fibroblast growth factor 4 (FGF-4), platelet-derived growth factor-ββ (PDGF-ββ), vascular endothelial growth factor-β (VEGF-β), and transforming growth factor-β1 (TGF-β1), with a control group receiving no supplementation. Growth curves were analyzed before and after cryopreservation, and gene expression of SCAPER (mitotic potential) and TP53 (apoptosis) was quantified using qPCR. As result, supplementation with FGF-2, FGF-4, and PDGF-ββ significantly enhanced cAM-MSC proliferation without altering the expression of proliferation-associated genes. These findings suggest that growth factor supplementation may be a viable strategy to improve cell viability in long-term culture and post-thaw recovery. |
| Authors |
Matheus Ferreira de Almeida  , Priscilla Avelino Ferreira Pinto , Lina Castelo Branco Motta , Tiago Gonçalves do Santos , Meline de Paula Coutinho , Jennifer Jullie Pichinelli Noronha , Vitória Mattos Pereira  , Sarah Ingrid Pinto Santos , Carlos Eduardo Ambrósio
|
| Journal Info |
Elsevier BV | Cryobiology , vol: 119
, pages: 105241 - 105241
|
| Publication Date |
3/28/2025 |
| ISSN |
0011-2240 |
Type |
article |
| Open Access |
closed
|
| DOI |
https://doi.org/10.1016/j.cryobiol.2025.105241 |
| Keywords |
Vitrification (Score: 0.4521055)
|
