| Abstract |
Cell lines derived from Spodoptera frugiperda (Sf), which are the most widely used hosts in the baculovirus-insect cell system, are contaminated with Sf-rhabdoviruses (Sf-RVs). In this study, we identified a closely related virus (Sf-CAT-RV) in the caterpillar species used to isolate the original Sf cell line. We then evaluated the Sf-RV and Sf-CAT-RV host ranges, found Sf-CAT-RV could infect Vero cells, and obtained results suggesting both variants can infect mouse ear fibroblasts. In addition, we found both variants could establish pantropic infections in severely immunocompromised (RAG2/IL2RG-/-) mice. However, both variants were cleared by two weeks post-inoculation and neither produced any symptoms or obvious adverse outcomes in these hosts. We conclude the caterpillars used to isolate Sf21 cells were the most likely source of the Sf-RV contaminant, Sf-RVs and their Sf-CAT-RV progenitor have broader host ranges than expected from previous work, but neither variant poses a serious threat to human health. |
| Authors |
Mark T. Menghini  , Christoph Geisler , Ajay B. Maghodia , Hoai J. Hallam , Steven L. Denton , Jason P. Gigley  , Donald L. Jarvis
|
| Journal Info |
Elsevier BV | Virology , vol: 585
, pages: 164 - 178
|
| Publication Date |
8/1/2023 |
| ISSN |
0042-6822 |
Type |
article |
| Open Access |
closed
|
| DOI |
https://doi.org/10.1016/j.virol.2023.05.007 |
| Keywords |
Plant-insect interactions (Score: 0.448387)
|
