| Abstract |
Mammals typically have hundreds of distinct olfactory sensory neuron subtypes, each defined by expression of a specific odorant receptor gene, which undergo neurogenesis throughout life at rates that can depend on olfactory experience. Here, we present a protocol to quantify the birthrates of specific neuron subtypes via the simultaneous detection of corresponding receptor mRNAs and 5-ethynyl-2′-deoxyuridine. For preparation prior to beginning the protocol, we detail procedures for generating odorant receptor-specific riboprobes and experimental mouse olfactory epithelial tissue sections. For complete details on the use and execution of this protocol, please refer to van der Linden et al. (2020).1 |
| Authors |
Kawsar Hossain  , Madeline Smith , Stephen W. Santoro 
|
| Journal Info |
Elsevier BV | STAR Protocols , vol: 4
, iss: 3
, pages: 102432 - 102432
|
| Publication Date |
9/1/2023 |
| ISSN |
2666-1667 |
Type |
article |
| Open Access |
gold
|
| DOI |
https://doi.org/10.1016/j.xpro.2023.102432 |
| Keywords |
Olfactory Receptors (Score: 0.581) , Olfactory System (Score: 0.536578)
|
