| Abstract |
Humans and animals encounter a summation of exposures during their lifetime (the exposome). In recent years, the scope of the exposome has begun to include microplastics. Microplastics (MPs) have increasingly been found in locations, including in animal gastrointestinal tracts, where there could be an interaction with Salmonella enterica serovar Typhimurium, one of the commonly isolated serovars from processed chicken. However, there is limited knowledge on how gut microbiomes are affected by microplastics and if an effect would be exacerbated by the presence of a pathogen. In this study, we aimed to determine if acute exposure to microplastics in vitro altered the gut microbiome membership and activity. The microbiota response to a 24 h co-exposure to Salmonella enterica serovar Typhimurium and/or low-density polyethylene (PE) microplastics in an in vitro broiler cecal model was determined using 16S rRNA amplicon sequencing (Illumina) and untargeted metabolomics. Community sequencing results indicated that PE fiber with and without S. Typhimurium yielded a lower Firmicutes/Bacteroides ratio compared with other treatment groups, which is associated with poor gut health, and overall had greater changes to the cecal microbial community composition. However, changes in the total metabolome were primarily driven by the presence of S. Typhimurium. Additionally, the co-exposure to PE fiber and S. Typhimurium caused greater cecal microbial community and metabolome changes than either exposure alone. Our results indicate that polymer shape is an important factor in effects resulting from exposure. It also demonstrates that microplastic-pathogen interactions cause metabolic alterations to the chicken cecal microbiome in an in vitro chicken cecal mesocosm. |
| Authors |
Chamia C. Chatman  , Elena G. Olson , Allison J. Freedman , Dana K. Dittoe  , Steven C. Ricke , Erica L.‐W. Majumder
|
| Journal Info |
American Society for Microbiology | Applied and Environmental Microbiology , vol: 90
, iss: 8
|
| Publication Date |
7/10/2024 |
| ISSN |
0099-2240 |
Type |
article |
| Open Access |
closed
|
| DOI |
https://doi.org/10.1128/aem.00915-24 |
| Keywords |
Metabolome (Score: 0.8951032) , Salmonella enterica (Score: 0.81745696) , Resistome (Score: 0.55732775) , Clostridia (Score: 0.54716164) , Fusobacteria (Score: 0.49983) , Indoor bioaerosol (Score: 0.42723873)
|
